Net N mineralization in the MELNHE study at Hubbard Brook Experimental Forest, Bartlett Experimental Forest and Jeffers Brook, central NH USA, 2008 - present
TITLE
Net N mineralization in the MELNHE study at Hubbard Brook Experimental Forest, Bartlett Experimental Forest and Jeffers Brook, central NH USA, 2008 - present
PRINCIPAL INVESTIGATOR(s)
Melany Fisk
Miami University
Biology Department Pearson Hall
Oxford, OH 45056
US
ABSTRACT:
The Multiple Element Limitation in Northern Hardwood Ecosystems (MELNHE) project studies N and P acquisition and limitation through a series of nutrient manipulations in northern hardwood forests. This data set includes net N mineralization measured in Oe, Oa, and mineral soil horizons in all 13 of the MELNHE study sites. Samples are collected every several years, beginning with pretreatment (2008 and 2009) through 2014, representing 3 years of N and P fertilization.
KEYWORD SET: Hubbard Brook Ecosystem Study LTER
Bartlett Experimental Forest, HBEF Watershed 101, HBR, Hubbard Brook LTER, Jeffers Brook, mineral horizon, net N mineralization, nitrogen fertilization, nitrification, Oa horizon, Oe horizon, phosphorous fertilization, soil.
KEYWORD SET: LTER Core Research Areas
disturbance, inorganic nutrients.
BEGIN DATE
2009
END DATE
2014
LOCATION
HBEF 101 MELNHE Plots
West bounding coordinate: -71.7404
East bounding coordinate: -71.7369
North bounding coordinate: 43.9390
South bounding coordinate: 43.9355
Jeffers Brook MELNHE Plots
West bounding coordinate: -71.8910
East bounding coordinate: -71.8880
North bounding coordinate: 44.0380
South bounding coordinate: 44.0340
Barlett MELNHE Plots
West bounding coordinate: -71.3230
East bounding coordinate: -71.2650
North bounding coordinate: 44.0610
South bounding coordinate: 44.0360
MELNHE Overview
Although temperate forests are generally thought of as N-limited, resource optimization theory predicts that ecosystem productivity should be co-limited by multiple nutrients. These ideas are represented in the Multi-Element Limitation (MEL) model (Rastetter et al. 2012). To test the patterns of resource limitation predicted by MEL, we are conducting nutrient manipulations in three study sites in New Hampshire: Bartlett Experimental Forest, Hubbard Brook Experimental Forest, and Jeffers Brook in the White Mountain National Forest. Additional project information can be found at http://www.esf.edu/melnhe/default.htm
Site Description
At Bartlett, we have three replicate stands of three ages, young (~20 y), mid-aged (30), and mature (>100 years). We have identified stands at Hubbard Brook and Jeffers Brook that corresponds to the mid-aged and mature stands at Bartlett, for 4 more stands. Each of the 13 stands has four 1/4 ha (50 m x 50 m) treatment plots, treated annually beginning in spring 2011, with N (30 kg N/ha/yr as NH4NO3), P (10 kg P/ha/yr as NaH2PO4), N+P, or nothing (an untreated control). Five stands also have a Ca treatment plot (1150 kg Ca/ha in the form of CaSiO3).
We are monitoring stem diameter, leaf area, sap flow, foliar chemistry, leaf litter production and chemistry, foliar nutrient resorption, root biomass and production, mycorrhizal associations, soil respiration, heterotrophic respiration, N and P availability, N mineralization, soil phosphatase activity, soil carbon and nitrogen, nutrient uptake capacity of roots, and mineral weathering. This data set includes N mineralization data.
Sampling Design
In 2008 and 2009 (pretreatment), approximately 30, 2-cm diameter soil cores were collected throughout each plot and composited by horizon in order to best represent the average condition of each plot. Beginning in 2010 (also pretreatment), we established 4 soil sampling subplots in each plot in order to confine the disturbance associated with sampling and at the same time ensure that repeated sampling was always done in the same general area. In 2010 and thereafter 3-4 soil cores were collected in each soil sampling subplot and divided into horizons (Oe, Oa, 10 cm of mineral). All cores in one plot (12-16 total, from the 4 subplots) were mixed together within each horizon and homogenized for one composite sample per horizon per plot. The mineral soil horizon was not sampled in all years.
Laboratory Procedures
Samples were refrigerated until processing, and were processed within 4 days of collection (usually processed the day after collection). Samples were homogenized by gentle separation of organic fragments over a 4-mm sieve followed by thorough mixing. Fragments > 4 mm were not included. Soil moisture content was determined by drying subsamples at 60 degrees C to constant mass. Time-zero subsamples were extracted in 2M KCl, allowed to settle overnight (12-16 hr), and filtered. Paired subsamples were incubated in mason jars at 15-18 degrees C for approximately 21 days, and then were extracted in the same manner as time-zero samples. Time-zero and incubated soil extracts were analyzed for NH4+ and NO3- concentrations using a Lachat autoanalyzer.
Calculations
Net N mineralization was calculated as the difference between incubated and time-zero NH4+ plus NO3- concentrations per g dry soil per day, and net nitrification was calculated as the difference between incubated and time-zero NO3- concentrations per g dry soil per day.
REFERENCES
  • Bae, B., Fahey, T.J., Yanai, R.D., and Fisk, M.C. 2015. Soil nitrogen availability affects belowground carbon allocation and soil respiration in northern hardwood forests of New Hampshire. Ecosystems 18(7):1179-1191.
  • Fisk, M.C., Ratliff, T.J., Goswami, S., and Yanai, R.D. 2014. Synergistic soil response to nitrogen plus phosphorus fertilization in hardwood forests. Biogeochemistry 118:195-204.
  • Rastetter, E.B., Yanai, R.D., Thomas, R.Q., Vadeboncoeur, M.A., Fahey, T.J., Fisk, M.C., Kwiatkowski, B.L., and Hamburg, S.P. 2013. Recovery from Disturbance Requires Resynchronization of Ecosystem Nutrient Cycles. Ecological Applications 23:621-642.
  • See, C.R., Yanai, R.D., Fisk, M.C., Vadeboncoeur, M.A., Quintero, B.A., and Fahey, T.J. 2015. Soil nitrogen affects phosphorus recycling: foliar resorption and plant-soil feedbacks in a northern hardwood forest. Ecology 96:2488–2498.
DATA ACCESS GUIDELINES
Data Use Policy



The re-use of scientific data has the potential to greatly increase communication, collaboration and synthesis within and among disciplines, and thus is fostered, supported and encouraged. Permission to use this dataset is granted to the Data User free of charge subject to the following terms:

1) Acceptable use. Use of the dataset will be restricted to academic, research, government or other not-for-profit professional purposes.

2) Redistribution. The data and metadata are provided for use by the Data User. The Data User will not redistribute the original Data Set or metadata to others without the explicit permission of the Principal Investigator.

3) Citation. It is considered a matter of professional ethics to acknowledge the work of other scientists. Thus, the Data User will properly attribute the Data Set in any publications or in the metadata of any derived data products that were produced using the Data Set. Citation should take the following general form: Creator, Year of Data Publication, Title of Dataset, Publisher, Dataset identifier.

Citation example: Holmes, R.T. 2012. Bird Abundances at Hubbard Brook (1969-2010) and on three replicate plots (1986-2000) in the White Mountain National Forest. Durham, NH. Hubbard Brook Data Archive [Database]. http://hubbardbrook.org/data/dataset.php?id=81 (23 July 2012)

4) Acknowledgment: The Data User should acknowledge any institutional support or specific funding awards referenced in the metadata accompanying this dataset in any publications where the Data Set contributed to its content. Acknowledgments should identify the supporting party, the party that received the support, and any identifying information such as grant numbers.

Acknowledgment example: Data on [topic] were provided by [name of PI] on [date]. These data were gathered as part of the Hubbard Brook Ecosystem Study (HBES). The HBES is a collaborative effort at the Hubbard Brook Experimental Forest, which is operated and maintained by the USDA Forest Service, Northern Research Station, Newtown Square, PA. Significant funding for collection of these data was provided by [agency]-[grant number], [agency]-[grant number], etc.

5) Consultation and questions. Data users are strongly encouraged to consult with the Principal Investigator(s) who collected these data for further information. Also, when appropriate, Data Users should consider including the Principal Investigator as a collaborator and/or co-author in the use of these data.

6) Notification. The Data User will notify the Principal Investigator of any publication or derivative work based on the Data Set. The Data User will also provide the Principal Investigator and/or the administrator of the Hubbard Brook Ecosystem Study with a pdf or two reprints of any publication(s) resulting from use of the Data Set.

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CONTACT PERSON

Information Manager, Hubbard Brook LTER
234 Mirror Lake Road
North Woodstock, NH 03262
USA

Phone: (603) 726-8902
Email: hbr-im@lternet.edu

Data file: melnhe_nmin.txt
Description: Nitrogen mineralization at MELNHE sites
Notes on Data: Data are stored at the Fisk lab at Miami University and on the MELNHE website.
ColumnVariableDescriptionUnitsCoded?Missing value label
1SiteResearch sitenoneynone
2AgeStand Agenoneynone
3StandStand namenoneynone
4PlotPlot numbernoneynone
5TreatmentFertilization treatmentnoneynone
6YearYear of sample collection.YYYYnnone
7DateDate of sample collection.YYYY-MM-DDnnone
8Days_IncLength of soil incubation in days.numbernnone
9OeNminNet N mineralization rate in the Oe horizon as ug N/g soil/daymicrogramPerGramPerDayn
-999.99
10OaNminNet N mineralization rate in the Oa horizon as ug N/g soil/daymicrogramPerGramPerDayn
-999.99
11MinNminNet N mineralization rate in the surface 10 cm of mineral soil as ug N/g soil/daymicrogramPerGramPerDayn
-999.99
12OeNitrNet nitrification rate in the Oe horizon as ug N/g soil/daymicrogramPerGramPerDayn
-999.99
13OaNitrNet nitrification rate in the Oa horizon as ug N/g soil/daymicrogramPerGramPerDayn
-999.99
14MinNitrNet nitrification rate in the surface 10 cm of mineral soil as ug N/g soil/daymicrogramPerGramPerDayn
-999.99
15OeNO3iNO3 concentration in time-zero extracts from the Oe horizon as ug N/g soilmicrogramPerGramn
-999.99
16OaNO3iNO3 concentration in time-zero extracts from the Oa horizon as ug N/g soilmicrogramPerGramn
-999.99
17MinNO3iNO3 concentration in time-zero extracts from the surface 10 cm of mineral soil as ug N/g soilmicrogramPerGramn
-999.99
18OeTNiNH4 plus NO3 concentration in time-zero extracts from the Oe horizon as ug N/g soil microgramPerGramn
-999.99
19OaTNiNH4 plus NO3 concentration in time-zero extracts from the Oa horizon as ug N/g soilmicrogramPerGramn
-999.99
20MinTNiNH4 plus NO3 concentration in time-zero extracts from the surface 10 cm of mineral soil as ug N/g soilmicrogramPerGramn
-999.99

CODES

Variable: Site
Code
Description
Bartlett
Bartlett Experimental Forest
W101
Hubbard Brook Experimental Forest Watershed 101
JB
Jeffers Brook
Variable: Age
Code
Description
Mature
100 years old
Mid
30 years old
Young
20 years old
Variable: Stand
Code
Description
C1
Stand C1 Bartlett Experimental Forest
C2
Stand C2 Bartlett Experimental Forest
C3
Stand C3 Bartlett Experimental Forest
C4
Stand C4 Bartlett Experimental Forest
C5
Stand C5 Bartlett Experimental Forest
C6
Stand C6 Bartlett Experimental Forest
C7
Stand C7 Bartlett Experimental Forest
C8
Stand C8 Bartlett Experimental Forest
O
Mature stand, Hubbard Brook Watershed 101 or Jeffers Brook
M
Mid-aged stand, Hubbard Brook Watershed 101 or Jeffers Brook
Variable: Plot
Code
Description
1
Plot 1 of 4 within a stand
2
Plot 2 of 4 within a stand
3
Plot 3 of 4 within a stand
4
Plot 4 of 4 within a stand
Variable: Treatment
Code
Description
none
no treatment
N
Nitrogen fertilization
NP
Nitrogen and phosphorous fertilization
P
Phosphorous fertilization
Con
Control plot

MISSING VALUE CODES
Variable
Missing Value Code
Code Explanation
OeNmin
-999.99
Data not available
OaNmin
-999.99
Data not available
MinNmin
-999.99
Data not available
OeNitr
-999.99
Data not available
OaNitr
-999.99
Data not available
MinNitr
-999.99
Data not available
OeNO3i
-999.99
Data not available
OaNO3i
-999.99
Data not available
MinNO3i
-999.99
Data not available
OeTNi
-999.99
Data not available
OaTNi
-999.99
Data not available
MinTNi
-999.99
Data not available